Ticks infesting cattle in the Central Equatoria region of South Sudan

Note: †, 1953–2011 Ticks infesting cattle represent a serious problem for improvement of cattle productivity in South Sudan. There has been limited information on ticks and tick-borne diseases in southern Sudan. This study was initiated to update the current distribution of ticks infesting cattle in the Central Equatoria region of South Sudan. The surveys for the present study were conducted at various cattle camps in Juba, Mangalla and Terekeka between December 2004 and June 2005. A total of 2322 ticks were collected from the bodies of 88 randomly selected cattle. Ticks were preserved in 70% ethanol for later identification. Seven ixodid tick species were found to infest cattle in Juba whilst six species were recorded in Mangalla and only four species in Terekeka. Amblyomma variegatum was the most common and widely distributed species found on cattle across all the study locations. Amblyomma lepidum was not found during this study. Based on these findings, it would be advisable to preempt the situation and institute containment procedures before possible East Coast fever outbreaks occur.

There has been limited information on ticks and tick-borne diseases in southern Sudan.The most detailed study is that of Hoogstraal (1956), which confirmed the presence of some of the most economically important tick species, including R. appendiculatus.Morzaria et al. (1981) reported the occurrence of R. appendiculatus in Chukudum, Aswa River, Palataka, Nimule and Juba.Julla (1994) reported that R. appendiculatus was abundant in and around Juba, while Malak et al. (2006) encountered the presence of R. appendiculatus during a participatory epidemiology study in the Kajo Kaji and Yei River counties.More recently, Marcellino (2008) encountered the presence of R. appendiculatus in and around Juba.
The total annual rainfall and topography determine the natural vegetation that covers the region.The ecological zones vary from savanna woodland, green belt and thick forest in the south to tropical rain forest in the north, which covers most of the region.Multiple species of evergreen trees and tall grass, with a south-to-north transition of forest, are present.The Bahr-el Jebel River crosses the region from south to north and divides it into two almost equal parts.There are many seasonal streams that flow from the central and south-western hills towards the main streams.
The region is rich in wildlife, with many small and large game species occurring mostly in the open savanna grassland.Their seasonal movement, commencing with the start of the rains, is towards Ethiopia, Kenya and Uganda.A variety of wildlife, including the African buffalo (Syncerus caffer), are found in three established national parks and six game reserves in South Sudan.African buffalo are known reservoir hosts of Theileria parva.
This study was initiated to update the current distribution of ticks infesting cattle in the Central Equatoria region of South Sudan.

Materials and methods
The surveys for the present study were conducted at various cattle camps in Juba, Mangalla and Terekeka between December 2004 and June 2005.Ticks were collected from cattle at Kansuk, Logo West, Tokiman (both eastern and western divisions), Gumbo, Kamiro, Kworijic and Nyambulung in the Juba district.In Mangalla samples were collected from cattle in the vicinity of the towns Mangalla and Malang, whilst in Terekeka samples were collected from cattle camps in the villages Terekeka, Lujura, Tukoro and Yari (Figure 1).
All the sites are stocked with the indigenous Nilotic Zebu cattle breed, which is kept under traditional management systems.This involves all cattle being tethered at night, which aided in the sampling.
All visible adult ticks were collected from the bodies of 88 randomly selected cattle using a pair of blunt forceps.Each animal was cast and restrained and particular attention was paid to predilection sites specific to different tick species, such as the ears, briskets, dewlap, perineum, udder, hump, inner hind legs, eyes and tail.Collected ticks were preserved in 70% ethanol in labelled vials for transport to the laboratory.Sampling records included a reference number for each tick and the date and locality of sampling.Ticks were identified according to the methods of Hoogstraal (1956), Okello-Onen, Hassan and Essuman (1999) and Walker et al. (2003).

Results
A total of 2322 ticks were collected from 88 animals at 14 sites in Juba, Mangalla and Terekeka (Table 1).Seven tick species from four ixodid genera were identified.These included A. variegatum, Hyalomma rufipes, R. (B.) decoloratus, R. appendiculatus, R. evertsi evertsi, R. sanguineous group, and R. simus.Amblyomma variegatum was found across the study area.Rhipicephalus appendiculatus, however, was abundant only in the Juba area; only one male specimen was identified from Mangalla and none was found in Terekeka.

Discussion
Ticks and tick-borne diseases are widespread (Food and Agriculture Organization 1983), but are most prevalent and Page 2 of 5 Theilleriosis Study Area Juba-area.shpJuba-rivers.shpexert their greatest impact in the tropical and subtropical regions of the world (Bram 1983).In many countries they present a major health impediment to the development and improvement of livestock.In addition to being efficient disease vectors, ticks may cause direct economic losses to livestock owing to damage caused by blood loss, hide, skin and teat damage, production and weight loss, and increased control costs.
Approximately 68 ixodid tick species have been recorded in Sudan (FAO 1983;Hoogstraal 1956;Jongejan et al. 1987;Karrar, Kaiser & Hoogstraal 1963;Osman 1978).The seven species found during this study have also previously been identified by Hoogstraal (1956), Morzaria et al. (1981) and Julla (1994) in Chukudum and Malak et al. (2006) in the Kajo Kaji and Yei counties.However, in the current survey, no specimens of R. appendiculatus were found in Terekeka during any of the two collections (May 2005 and June 2005) and only one male specimen was recorded in Mangalla.Amblyomma variegatum, the tropical bont tick, was the most common and widely distributed species found on cattle across all the study locations.Previously, this species was reported to infest cattle, sheep, goats and buffaloes.Its adult stage is known to be present throughout the year, with heavier infestations in the rainy season (Walker et. al. 2003).At Juba, the most abundant tick species were A. variegatum and R. appendiculatus.Rhipicephalus sanguineus group and R. simus were found infesting only cattle at Board Yard and Gumbo cattle camps although they are known to infest domestic dogs.Rhipicephalus simus occurs in savanna regions and has never been encountered in very large numbers (Walker et al. 2003).Seven ixodid tick species were found to infest cattle in Juba whilst six species were recorded in Mangalla and only four species in Terekeka.Amblyomma variegatum, H. rufipes, R (B.) decoloratus and R. evertsi evertsi were prevalent in the whole study area.Amblyomma lepidum was not found during this study, but was reported in previous studies to be rare in the area and mainly found on cattle at slaughterhouses (Julla 1994).
Although only one male R. appendiculatus specimen was found, the high prevalence of antibodies against T. parva in cattle in Mangalla (Marcellino 2008) suggests that the distributional area of East Coast fever may extend northwards to Bor.This may be related to Dinka Bor cattle having moved through the Juba-Mangalla area, where the disease is endemic, on their way back home from the West Equatoria region following the signing of comprehensive peace agreements in January 2005.The prevalence of T. parva antibodies and its vector, R. appendiculatus, in this area is contrary to the prediction of Morzaria et al. (1981) that R. appendiculatus occurs in a cool climate with high rainfall and generally does not tolerate daily maximum temperatures higher than 30 °C or a dry season longer than four months.The climate of Juba does not appear to be conducive to its establishment and maintenance.In fact, further north of Juba conditions became more arid.The spread of the tick vector is further supported by studies of Lessard et al. (1990), in which the computer model Climex predicted that most of southern Sudan is suitable for the establishment and maintenance of R. appendiculatus.However, the northern limit of its distribution is still not very clearly delineated; the present study extended only 110 km north of Juba.Based on these findings, it would be advisable to preempt the situation and institute containment procedures before possible East Coast fever outbreaks occur.In addition, with regard to the epidemiology of theileriosis in general, and T. parva in particular, antibodies to T. parva in cattle from the Bahr el Ghazal region have been demonstrated by Zessein and Bauman (1982).Detailed epidemiological studies are needed to confirm the absence or presence of R. appendiculatus in the area.The absence of R. appendiculatus in Terekeka during the present survey may be attributed to overstocking and subsequent overgrazing, resulting in reduced vegetation cover.Hornby (1935) found that grass cover correlated positively with numbers of R. appendiculatus in Tanzania, where overgrazing and overstocking had resulted in reduced numbers of ticks due to a lack of grass cover.These observations have also been endorsed by studies of Yeoman andWalker (1967), Norval (1977) and Short et al. (1989).
A wider distribution of R. appendiculatus is expected to have a serious impact on the future of cattle improvement programmes in areas endemic to theileriosis.A systematic survey involving monthly collection of ticks throughout the year is required in Terekeka to gain epidemiological data relevant to theileriosis in the area.

Conclusion
Seven ixodid tick species were found to infest cattle in Juba whilst six species were recorded in Mangalla and only four species in Terekeka.Amblyomma variegatum, the tropical bont tick, was the most common and widely distributed species found on cattle across all the study locations.Amblyomma lepidum was not found during this study.At Juba, the most abundant tick species were A. variegatum and R. appendiculatus.Rhipicephalus sanguineus group and R. simus were found infesting only cattle at Board Yard and Gumbo cattle camps although they are known to infest domestic dogs.Based on these findings, it would be advisable to preempt the situation and institute containment procedures before possible East Coast fever outbreaks occur.

FIGURE 1 :
FIGURE 1: The Central Equatoria region of southern Sudan, indicating sampling localities.

TABLE 1 :
Numbers of ticks collected on cattle at selected locations in the Central Equatoria region, Sudan (December 2004 and January-June 2005).

TABLE 2 :
Tick loads at selected locations in the Central Equatoria region, Sudan (December 2004; January-June 2005)., Tick load is defined as the number of ticks collected per number of animals sampled. a