Original Research

Immunogenicity of a plasmid DNA vaccine encoding G1 epitope of bovine ephemeral fever virus G glycoprotein in mice

Reza Pasandideh, Masoud Reza Seyfi Abad Shapouri, Mohammad Taghi Beigi Nassiri
Onderstepoort Journal of Veterinary Research | Vol 85, No 1 | a1617 | DOI: https://doi.org/10.4102/ojvr.v85i1.1617 | © 2018 Reza Pasandideh, Masoud Reza Seyfi Abad Shapouri, Mohammad Taghi Beigi Nassiri | This work is licensed under CC Attribution-NoDerivatives 4.0
Submitted: 24 February 2018 | Published: 28 August 2018

About the author(s)

Reza Pasandideh, Department of Animal Science, Khuzestan Agricultural Sciences and Natural Resources University, Ahvaz, Iran, Islamic Republic of
Masoud Reza Seyfi Abad Shapouri, Department of Pathobiology, Shahid Chamran University of Ahvaz, Iran, Islamic Republic of
Mohammad Taghi Beigi Nassiri, Department of Animal Science, Khuzestan Agricultural Sciences and Natural Resources University, Ahvaz, Iran, Islamic Republic of


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Abstract

The aim of this study was to investigate the immunogenicity of a plasmid deoxyribonucleic acid (DNA) vaccine encoding the G1 epitope of bovine ephemeral fever virus (BEFV) G glycoprotein in mice. A plasmid DNA carrying the G1 gene was constructed and designated as pcDNA3.1-G1. The expression of the target gene was confirmed in human embryonic kidney 293 (HEK 293) cells transfected with pcDNA3.1-G1 by indirect immunofluorescent staining. Immunisation experiments were intramuscularly carried out by vaccinating 6-week-old female mice in four groups, including the pcDNA3.1-G1 construct, pcDNA3.1 (+) plasmid alone, BEF-inactivated vaccine and phosphate-buffered saline (PBS) (1X) three times with 2-week intervals. Fourteen days after the last immunisation, the animals were bled and the resulting sera were tested for anti-G1-specific antibodies by immunoblotting analysis, indirect enzyme-linked immunosorbent assay (ELISA) and virus neutralisation (VN) test. Serological assays showed that the pcDNA3.1-G1 construct expressing G1 protein was able to elicit specific antibodies against this antigen. Virus neutralisation test showed that pcDNA3.1-G1 could induce anti-BEFV-neutralising antibodies in mice. Our findings indicated that a new dimension can be added to vaccine studies for bovine ephemeral fever (BEF) using eukaryotic expression plasmids encoding the G1 antigen in the future.

Keywords

Bovine ephemeral fever virus (BEFV); DNA vaccine; G1 antigen

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