Original Research

Failure of diplodiatoxin to induce diplodiosis in juvenile goats

Christo J. Botha, Louis G.J. Ackerman, Mxolisi G. Masango, Luke F. Arnot
Onderstepoort Journal of Veterinary Research | Vol 87, No 1 | a1712 | DOI: https://doi.org/10.4102/ojvr.v87i1.1712 | © 2020 Christo J. Botha, Louis G.J. Ackerman, Mxolisi G. Masango, Luke F. Arnot | This work is licensed under CC Attribution-NoDerivatives 4.0
Submitted: 09 November 2018 | Published: 05 March 2020

About the author(s)

Christo J. Botha, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa
Louis G.J. Ackerman, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa
Mxolisi G. Masango, Analytics and Institutional Research Unit, University of the Witwatersrand, Johannesburg, South Africa
Luke F. Arnot, Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa


Share this article

Bookmark and Share

Abstract

Diplodiosis is an important neuromycotoxicosis of ruminants in South Africa when grazing on harvested maize fields in winter. It is believed to be caused by mycotoxin(s) synthesised by Stenocarpella (Diplodia) maydis. Although several metabolites have been isolated from S. maydis culture material, none of these have been administered to ruminants to reproduce the disease. The objectives of this study were to isolate diplodiatoxin and to administer it to juvenile goats. Diplodiatoxin, considered as a major metabolite, was purified from S. maydis-infected maize cultures (Coligny 2007 isolate). Following intravenous administration of 2 mg and 4 mg diplodiatoxin/kg body weight for five consecutive days to two juvenile goats, no clinical signs reminiscent of diplodiosis were observed. Based on previous experimental results and if diplodiatoxin was the causative compound, the dosage regimen employed was seemingly appropriate to induce diplodiosis. In addition, intraruminal administration of 2 mg/kg diplodiatoxin to one goat for three consecutive days also did not induce clinical signs. It appears as if diplodiatoxin alone is not the causative compound. Other metabolites and/or mixtures of diplodiatoxin and other mycotoxins, when available in sufficient quantities, should also be evaluated.

Keywords

diplodiosis; diplodiatoxin; Stenocarpella maydis; mycotoxin; neuromycotoxicosis

Metrics

Total abstract views: 1032
Total article views: 458


Crossref Citations

No related citations found.