Original Research

Molecular and restriction fragment length polymorphism analysis of canine parvovirus 2 (CPV-2) in dogs in southeast Anatolia, Turkey

Pelin F. Polat, Adem Şahan, Gürbüz Aksoy, Mehmet O. Timurkan, Ender Dinçer
Onderstepoort Journal of Veterinary Research | Vol 86, No 1 | a1734 | DOI: https://doi.org/10.4102/ojvr.v86i1.1734 | © 2019 Pelin F. Polat, Adem Şahan, Gürbüz Aksoy, Mehmet O. Timurkan, Ender Dinçer | This work is licensed under CC Attribution-NoDerivatives 4.0
Submitted: 29 January 2019 | Published: 27 August 2019

About the author(s)

Pelin F. Polat, Department of Internal Medicine, Faculty of Veterinary Medicine, Harran University, Sanliurfa, Turkey
Adem Şahan, Department of Internal Medicine, Faculty of Veterinary Medicine, Harran University, Sanliurfa, Turkey
Gürbüz Aksoy, Department of Internal Medicine, Faculty of Veterinary Medicine, Harran University, Sanliurfa, Turkey
Mehmet O. Timurkan, Department of Virology, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey
Ender Dinçer, Advanced Technology Education, Research and Application Center, Mersin University, Mersin, Turkey


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Abstract

Canine parvovirus-2 (CPV-2) is the aetiological agent of an infectious viral disease of dogs, characterised by diarrhoea and vomiting. Mutations of the CPV-2 genome have generated new variants circulating worldwide. This article reports the molecular analysis of CPV-2 variants collected in the dog population in southeast Anatolia, Turkey. Twenty blood samples previously taken for the laboratory diagnosis of dogs with suspected parvovirus were screened for CPV-2 by polymerase chain reaction (PCR). Of the 20 samples, 18 tested positive for CPV-2. Partial VP2 gene sequencing and restriction fragment length polymorphism (RFLP) analysis revealed CPV-2a (n = 1), CPV-2b (n = 16) and CPV-2c (n = 1) variants. Phylogenetic analysis based on the partial length VP2 gene showed that CPV-2b (n = 15) variants showed sequences clustering separately in the phylogenetic tree. The CPV-2c sample was phylogenetically related to Chinese strains and Indonesia strain, whereas the CPV-2a sample was phylogenetically related to the Portuguese strain. These results, which are the first to demonstrate the presence of CPV-2c in the dog population of southeast Anatolia, Turkey, indicate that CPV-2a/2b/2c variants co-exist in Turkey’s dog population.

Keywords

Canine Parvovirus variants; molecular characterisation; Turkey; RFLP; restriction fragment length polymorphism

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