Proceedings

Bartonella spp. in human and animal populations in Gauteng, South Africa, from 2007 to 2009

Anastasia N. Trataris, Jennifer Rossouw, Lorraine Arntzen, Allan Karstaedt, John Frean
Onderstepoort Journal of Veterinary Research | Vol 79, No 2 | a452 | DOI: https://doi.org/10.4102/ojvr.v79i2.452 | © 2012 Anastasia N. Trataris, Jennifer Rossouw, Lorraine Arntzen, Allan Karstaedt, John Frean | This work is licensed under CC Attribution 4.0
Submitted: 12 June 2012 | Published: 20 June 2012

About the author(s)

Anastasia N. Trataris, National Institute for Communicable Diseases, National Health Laboratory Service, South Africa
Jennifer Rossouw, National Institute for Communicable Diseases, National Health Laboratory Service, South Africa
Lorraine Arntzen, National Institute for Communicable Diseases, National Health Laboratory Service, South Africa
Allan Karstaedt, University of the Witwatersrand, Parktown, South Africa
John Frean, National Institute for Communicable Diseases, National Health Laboratory Service, South Africa

Abstract

Bartonellae are highly adaptive organisms that have the ability to evade the host immune system and cause persistent bacteraemia by occupying the host’s erythrocytes. Bartonella spp. is under-studied and health care professionals often misdiagnose Bartonella-related infections. The aim of this study was to investigate the carriage of Bartonella spp. circulating in human and animal populations in Gauteng using culturing and polymerase chain reaction (PCR) detection. A total of 424 human, 98 cat, 179 dog, and 124 wild rodent blood samples were plated onto specialised media and incubated for 7–21 days at 37 ºC in CO2. Culture isolates morphologically similar to Bartonella control strains were confirmed by PCR and sequenced to determine species. Deoxyribonucleic acid (DNA) was extracted from all blood samples and tested by nested PCR. Bartonella could only be cultured from the cat and rodent specimens. Cat isolates were > 99% similar to Bartonella henselae URBHLIE 9, previously isolated from an endocarditis patient, and rat isolates were > 98% similar to either RN24BJ (candidus ‘Bartonella thailandensis’) or RN28BJ, previously isolated from rodents in China. The PCR prevalences were 22.5% in HIV-positive patients, 9.5% in clinically healthy volunteers, 23.5% in cats, 9% in dogs and 25% in rodents. Findings of this study have important implications for HIV-positive patients.

Keywords

Bartonella prevalence; humans; cats; dogs and rodent population prevalence; culture prevalence; PCR prevalence

Metrics

Total abstract views: 6139
Total article views: 13226

 

Crossref Citations

1. Bartonella Bacteria in Urban Rats: A Movement From the Jungles of Southeast Asia to Metropoles Around the Globe
Michael Kosoy, Ying Bai
Frontiers in Ecology and Evolution  vol: 7  year: 2019  
doi: 10.3389/fevo.2019.00088