Article Information

Authors:
Itumeleng I. Setshedi1,2
Gerda Fouche1
John Dewar2
Vinesh Maharaj1
Martin S. Myer1,2

Affiliations:
1CSIR Bio-prospecting, Pretoria, South Africa

2Department of Life and Consumer Sciences, University of South Africa, South Africa

Correspondence to:
Itumeleng Setshedi

Postal address:
PO Box 3024, Morogoro, Tanzania

How to cite this poster:
Setshedi, I.I., Fouche, G., Dewar, J., Maharaj, V. & Myer, M.S., 2012, ‘Phytochemical isolation of compounds from Sceletium tortuosum and activity testing against Plasmodium falciparum’, Onderstepoort Journal of Veterinary Research 79(2), Art. #481, 1 page. http://dx.doi.org/10.4102/
ojvr.v79i2.481

Note:
Proceedings of the Conference of the Southern African Centre for Infectious Disease Surveillance ‘One Health’ held at the National Institute for Communicable Diseases, Johannesburg, July 2011.

Copyright Notice:
© 2012. The Authors. Licensee: AOSIS OpenJournals.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Phytochemical isolation of compounds from Sceletium tortuosum and activity testing against Plasmodium falciparum
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Malaria is a major health care problem in tropical regions due to the increasing resistance of Plasmodium falciparum against widely available antimalarial drugs. Traditional societies relied on medicinal plants to treat parasitic infections. As a result, drugs like quinine and artemisinin were isolated from herbs and barks (Varughese et al. 2010). Sceletium tortuosum has been used as medicine for social and spiritual purposes by San hunter gatherers and Khoi pastoralists. Sceletium tortuosum is rich in alkaloids, one of the important classes of natural product producing treatment for parasitic infections (Kayser et al. 2002).

Laboratory preparation of extracts of fresh S. tortuosum plant material was conducted mimicking traditional methods of preparation using organic solvents. Mesembrine was isolated from a methanol extract using conventional column chromatography. Sixteen extracts and mesembrine were evaluated for antiplasmodium activity using a plasmodium lactate dehydrogenase culture sensitivity assay with chloroquine as reference drug.

Of the sixteen extracts, four showed activity against P. falciparum with IC50 ranging between 1.47 µg/mL and 7.32 µg/mL. Extracts prepared from stored material at -20 °C showed no antiplasmodium activity. The four originally active extracts were re-screened six months later, but the antimalarial activity could not be reproduced. To determine discrepancy in biological results, chemical profiling of the extracts was done using high performance liquid chromatography technique. Differences were observed in the profiles of the active extracts when compared to those of stored plant material.

The instability of plant constituents observed could be a result of plant storage suggesting that the plant is best used when fresh.



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