Original Research

Construction of an artificial recombinant bicistronic plasmid DNA vaccine against porcine rotavirus

Tingting Cui, Jun Xiong, Yongzhi Wang, Xintian Wen, Xiaobo Huang, Yong Huang, Xiaoping Ma, Zhongkai Hu, Qin Zhao, Sanjie Cao
Onderstepoort Journal of Veterinary Research | Vol 80, No 1 | a498 | DOI: https://doi.org/10.4102/ojvr.v80i1.498 | © 2013 Tingting Cui, Jun Xiong, Yongzhi Wang, Xintian Wen, Xiaobo Huang, Yong Huang, Xiaoping Ma, Zhongkai Hu, Qin Zhao, Sanjie Cao | This work is licensed under CC Attribution-NoDerivatives 4.0
Submitted: 27 June 2012 | Published: 04 March 2013

About the author(s)

Tingting Cui, College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China
Jun Xiong, College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China
Yongzhi Wang, Sichuan Rural Science and Technology Development Centre, Chengdu, China
Xintian Wen, College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China
Xiaobo Huang, College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China
Yong Huang, College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China
Xiaoping Ma, College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China
Zhongkai Hu, College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China
Qin Zhao, College of Food Science, Sichuan Agricultural University, China
Sanjie Cao, College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China


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Abstract

The attenuated Salmonella typhimurium χ4550 strain was used to harbour a reconstructed bicistronic DNA vaccine against porcine rotavirus, which carried the rotavirus nonstructural protein 4 (NSP4) and VP7 genes simultaneously. Using a balanced lethal system, the kanamycin resistance gene of expressing eukaryotic plasmids pVAX1 and pVAXD were replaced by the aspartate β-semialdehyde dehydrogenase (asd) gene. The NSP4 cleavage product (259–525) of rotavirus OSU strain and VP7 full-length genes were amplified by reverse transcription polymerase chain reaction and then inserted into the eukaryotic single-expression plasmid, pVAX1-asd, and the eukaryotic dual-expression plasmid, pVAXD-asd, respectively. The recombinant plasmids pVAX1-asd-NSP4, pVAX1-asd-VP7 and pVAXD-asd-NSP4-VP7 were transformed into the attenuated S. typhimurium χ4550 strain by electrotransformation. An indirect immunofluorescence assay of the expressed COS-7 cell suggested that the recombinant S. typhimurium χ4550 strain was constructed successfully. The recombinant S. typhimurium χ4550 strain was orally administered to BALB/c mice. The group immunised with dual- expression plasmids produced a significantly higher level of serum Immunoglobulin G (IgG) and intestinal Immunoglobulin A (IgA) than the group immunised with single-expression plasmids. These results indicated that eukaryotic bicistronic plasmid DNA vaccines could be successfully constructed to enhance humoural, mucosal and cellular immune response against rotavirus infection.

Keywords

DNA vaccine; immune response; NSP4 gene; porcine rotavirus; Salmonella typhimurium χ4550 strain; VP7 gene

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