Original Research

Evaluation of a rapid immunodiagnostic test kit for detection of African lyssaviruses from brain material

W. Markotter, D. York, C.T. Sabeta, W. Shumba, G. Zulu, K. Le Roux, L.H. Nel
Onderstepoort Journal of Veterinary Research | Vol 76, No 2 | a50 | DOI: https://doi.org/10.4102/ojvr.v76i2.50 | © 2009 W. Markotter, D. York, C.T. Sabeta, W. Shumba, G. Zulu, K. Le Roux, L.H. Nel | This work is licensed under CC Attribution-NoDerivatives 4.0
Submitted: 09 September 2009 | Published: 09 September 2009

About the author(s)

W. Markotter,
D. York,
C.T. Sabeta,
W. Shumba,
G. Zulu,
K. Le Roux,
L.H. Nel,

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Abstract

Rapid immunodiagnostic test kit was evaluated against a selection of isolates of lyssavirus genotypes occurring in Africa. The test was carried out in parallel comparison with the fluorescent antibody test (FAT) and isolates representing previously established phylogenetic groups from each genotype were included. The specificity of the rapid immunodiagnostic test compared favourably with the FAT and was found to detect all representatives of genotypes 1, 2, 3 and 4 in brain samples of either field cases or suckling mouse brain inoculates.

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