Original Research

Histomorphometrical and ultrastructural study of the effects of carbendazim on the magnum of the Japanese quail (Coturnix coturnix japonica)

Wahabu H. Kimaro, Mary-Catherine Madekurozwa, Herman B. Groenewald
Onderstepoort Journal of Veterinary Research | Vol 80, No 1 | a579 | DOI: https://doi.org/10.4102/ojvr.v80i1.579 | © 2013 Wahabu H. Kimaro, Mary-Catherine Madekurozwa, Herman B. Groenewald | This work is licensed under CC Attribution 4.0
Submitted: 20 February 2013 | Published: 19 July 2013

About the author(s)

Wahabu H. Kimaro, Department of Anatomy and Physiology, University of Pretoria, South Africa and Department of Veterinary Anatomy, Sokoine University of Agriculture, Tanzania
Mary-Catherine Madekurozwa, Department of Anatomy and Physiology, University of Pretoria, South Africa
Herman B. Groenewald, Department of Anatomy and Physiology, University of Pretoria, South Africa

Abstract

The study investigated the effect of various doses of carbendazim on the morphology of the magnum of the Japanese quail. No morphological changes were observed in the magnum in birds treated with carbendazim at doses of 25 mg/kg and 100 mg/kg bodyweight. A carbendazim dose of 400 mg/kg bodyweight was the lowest dose which caused morphological changes in the magnum. Histologically, carbendazim caused pyknosis and glandular atrophy in the magnum mucosa. Carbendazim also caused significant decreases in the height of the mucosal folds, epithelial height, glandular width and glandular luminal diameter at 400 mg/kg and 800 mg/kg (p < 0.05). At ultrastructural level, dose-dependent deciliation was observed. Pyknotic nuclei, dilated cisternae of rough endoplasmic reticulum, swollen mitochondria, numerous vacuoles and lysosomes in the luminal and glandular epithelia were identified. The observed degenerative changes could be due to cytoskeletal disruption caused by carbendazim toxicity. Degeneration of the luminal and glandular cells in the magnum pose a potential threat to the egg production and reproduction of exposed birds.

Keywords

carbendazim; magnum; morphometry; ultrastructure; Japanese quail

Metrics

Total abstract views: 6003
Total article views: 14062

 

Crossref Citations

1. Theoretical design and preparation of ionic liquid-based magnetic nanoparticles for the magnetic dispersive solid-phase extraction of benzimidazoles in human plasma
Rui Chen, Xianzhao Zhang, Fengmao Liu, Chengcheng Liu, Qingrong Peng, Xiaoqiang Qiao
Separation and Purification Technology  vol: 302  first page: 122150  year: 2022  
doi: 10.1016/j.seppur.2022.122150

2. The effects of copper and L-arginine supplementation on sexual maturation, egg quality, blood parameters, and oviduct morphology in laying hens
Hasan Rouhanipour, Omid Ashayerizadeh, Seyed Davood Sharifi, Behrouz Dastar
Journal of Applied Poultry Research  vol: 35  issue: 2  first page: 100670  year: 2026  
doi: 10.1016/j.japr.2026.100670

3. Hydroxychloride trace elements improved eggshell quality partly by modulating uterus histological structure and inflammatory cytokines expression in aged laying hens
Qiuyu Jiang, Jingjing Sun, Yang He, Yanbo Ma, Bingkun Zhang, Yanming Han, Yuanyuan Wu
Poultry Science  vol: 100  issue: 11  first page: 101453  year: 2021  
doi: 10.1016/j.psj.2021.101453

4. Amino acid ionic liquid–based magnetic dispersive solid-phase extraction for benzimidazole residue analysis in fruit juice and human serum based on theoretical screening
Rui Chen, Xiaoqiang Qiao, Fengmao Liu, Xuehui Chen
Food Chemistry  vol: 404  first page: 134695  year: 2023  
doi: 10.1016/j.foodchem.2022.134695

5. Yeast culture promotes albumen quality by improving magnum protein secretion and intestinal microbiota in aged laying hens
Li‐bing Gao, Dong Dai, Peng Chen, Hai‐jun Zhang, Shu‐geng Wu, Guang‐hai Qi, Jing Wang
Journal of the Science of Food and Agriculture  vol: 105  issue: 12  first page: 6319  year: 2025  
doi: 10.1002/jsfa.14364

6. Multi-omics analysis reveals the molecular regulatory network underlying the prevention of Lactiplantibacillus plantarum against LPS-induced salpingitis in laying hens
Dan Song, Aike Li, Bingxu Chen, Jia Feng, Tao Duan, Junlin Cheng, Lixian Chen, Weiwei Wang, Yuna Min
Journal of Animal Science and Biotechnology  vol: 14  issue: 1  year: 2023  
doi: 10.1186/s40104-023-00937-x

7. Impact of pubertal imidacloprid exposure on the genital tract of Japanese quail (Coturnix Coturnix japonica): Histomorphometric and ultrastructural study
Mohammed I.A. Ibrahim, Rephima M. Phaswane, Antoinette V. Lensink, Christo J. Botha
Tissue and Cell  vol: 96  first page: 102997  year: 2025  
doi: 10.1016/j.tice.2025.102997

8. Salvia miltiorrhiza enhances laying performance, follicular development, and egg quality of aged laying hens: Mechanistic insights from in vivo and in silico studies
Chengwen Meng, Md Abul Kalam Azad, Wenchao Lin, Jue Gui, Yadong Cui, Wei Lan, Xiangfeng Kong
Journal of Agriculture and Food Research  vol: 23  first page: 102233  year: 2025  
doi: 10.1016/j.jafr.2025.102233

9. Effects of the pesticide carbendazim on broiler chicken health and carbendazim residue levels in broiler tissues
Wanjun Zhang, Yutong Tang, Ruiqi Fan, Fude Zhang, Yongchen Li, Jianjun Zang, Wenjun Yang, Zongyi Wang, Xiaoyan Yuan, Yuan Yang, Yiqiang Chen
Veterinary Research Communications  vol: 48  issue: 5  first page: 3131  year: 2024  
doi: 10.1007/s11259-024-10484-z

10. Individual and synergistic toxic effects of carbendazim and chlorpyrifos on zebrafish embryonic development
Ruiqi Fan, Wanjun Zhang, Lizhong Li, Li Jia, Jun Zhao, Zengming Zhao, Shuangqing Peng, Xiaoyan Yuan, Yiqiang Chen
Chemosphere  vol: 280  first page: 130769  year: 2021  
doi: 10.1016/j.chemosphere.2021.130769

11. Dietary tea polyphenol supplementation improved egg production performance, albumen quality, and magnum morphology of Hy-Line Brown hens during the late laying period1
Xiao-cui Wang, Xiao-hong Wang, Jing Wang, Hao Wang, Hai-jun Zhang, Shu-geng Wu, Guang-hai Qi
Journal of Animal Science  vol: 96  issue: 1  first page: 225  year: 2018  
doi: 10.1093/jas/skx007

12. Phytogenic Antioxidants Prolong n-3 Fatty Acid-Enriched Eggs’ Shelf Life by Activating the Nrf-2 Pathway through Phosphorylation of MAPK
Muhammad Suhaib Shahid, Shengyu Zhou, Wei Nie, Liang Wang, Huiyuan Lv, Jianmin Yuan
Foods  vol: 11  issue: 20  first page: 3158  year: 2022  
doi: 10.3390/foods11203158

13. Effects of Supplementing Selenium-Enriched Cardamine violifolia to Laying Hens on Egg Quality and Yolk Antioxidant Capacity during Storage at 4 °C and 25 °C
Kun Qin, Xin Cong, Hui Wang, Mengke Yan, Xianfeng Xu, Mingkang Liu, Fulong Song, Dan Wang, Xiao Xu, Jiangchao Zhao, Shuiyuan Cheng, Yulan Liu, Huiling Zhu
Foods  vol: 13  issue: 5  first page: 802  year: 2024  
doi: 10.3390/foods13050802

14. Changes in progesterone receptor isoforms expression and in the morphology of the oviduct magnum of mature laying and aged nonlaying hens
María Genoveva González-Morán
Biochemical and Biophysical Research Communications  vol: 478  issue: 2  first page: 999  year: 2016  
doi: 10.1016/j.bbrc.2016.08.071

15. Effect of different protein ingredients on performance, egg quality, organ health, and jejunum morphology of laying hens
X.C. Wang, H.J. Zhang, H. Wang, H.Y. Yue, J. Wang, S.G. Wu, G.H. Qi
Poultry Science  vol: 96  issue: 5  first page: 1316  year: 2017  
doi: 10.3382/ps/pew396