Research Communication

Studies towards optimising the isolation of diplonine, a neurotoxin isolated from cultures of Stenocarpella maydis (Berk.) Sacc.

Leendert D. Snyman, Bradley C. Flett, Rowina A. Schultz
Onderstepoort Journal of Veterinary Research | Vol 81, No 1 | a765 | DOI: https://doi.org/10.4102/ojvr.v81i1.765 | © 2014 Leendert D. Snyman, Bradley C. Flett, Rowina A. Schultz | This work is licensed under CC Attribution 4.0
Submitted: 30 January 2014 | Published: 12 November 2014

About the author(s)

Leendert D. Snyman, Department of Toxicology, Agricultural Research Council, Onderstepoort Veterinary Institute, South Africa
Bradley C. Flett, Crop Protection (Pathology), Agricultural Research Council, Grain Crop Institute, Potchefstroom, South Africa; Unit of Environmental Sciences and Development, North-West University, Potchefstroom Campus, South Africa
Rowina A. Schultz, Department of Toxicology, Agricultural Research Council, Onderstepoort Veterinary Institute, South Africa


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Abstract

Diplonine, a mycotoxin that induces neurotoxic clinical signs in the guinea pig, resembling those occurring in cattle and sheep with diplodiosis, was isolated previously from a Stenocarpella maydisculture. Knowledge of the chemical properties of the toxin, which was characterised as a substituted ß-cyclopropylamino acid, enabled amendments in the present study to the initial steps of the isolation procedure. Extraction with water and fractionation by cation exchange chromatography improved the efficiency of isolation, potentially allowing the preparation of larger amounts of the toxin.

Keywords

diplonine, diplodiosis, neuromycotoxin, Stenocarpella maydis

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