Original Research
Genetic diversity of Ehrlichia ruminantium strains in Cameroon
Onderstepoort Journal of Veterinary Research | Vol 81, No 1 | a840 |
DOI: https://doi.org/10.4102/ojvr.v81i1.840
| © 2014 Seraphine N. Esemu, Roland N. Ndip, Lucy M. Ndip
| This work is licensed under CC Attribution 4.0
Submitted: 27 June 2014 | Published: 25 November 2014
Submitted: 27 June 2014 | Published: 25 November 2014
About the author(s)
Seraphine N. Esemu, Laboratory for Emerging Infectious Diseases, University of Buea,, CameroonRoland N. Ndip, Department of Microbiology and Parasitology, University of Buea, Cameroon; Department of Biochemistry and Microbiology, University of Fort Hare, South Africa
Lucy M. Ndip, Laboratory for Emerging Infectious Diseases, University of Buea, Cameroon; Department of Biomedical Sciences, University of Buea, Cameroon; Center for Tropical Diseases, University of Texas Medical Branch, United States
Abstract
In order to investigate the extent of genetic diversity among Ehrlichia ruminantium strains in Cameroon, a partial fragment (800 bp) of the E. ruminantium map1 gene was amplified by nested polymerase chain reaction in 121 of 156 E. ruminantium pCS20-positive DNA samples extracted from ticks and cattle collected from two ranches. Deoxyribonucleic acid sequencing of the map1 gene products indicated the presence of at least 21 genotypes at the nucleotide level and 16 genotypes at the amino acid level circulating within the study sites. Some of the genotypes were identical to Antigua (U50830), Blaaukrans (AF368000) or UmBanein (U50835), whilst the others were new genotypes. Twenty-four representative sequences were deposited in GenBank and given accession numbers JX477663 – JX477674 (for sequences of tick origin) and JX486788 – JX486799 (for sequences of cattle origin). Knowledge of E. ruminantium strain diversity could be important in understanding the epidemiology of heartwater
Keywords
Cameroon; cattle; Ehrlichia ruminantium; genetic diversity; ticks
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