Original Research

The effect of Rift Valley fever virus Clone 13 vaccine on semen quality in rams

Geoff Brown, Estelle H. Venter, Paul Morley, Henry Annandale
Onderstepoort Journal of Veterinary Research | Vol 82, No 1 | a919 | DOI: https://doi.org/10.4102/ojvr.v82i1.919 | © 2015 Geoff Brown, Estelle H. Venter, Paul Morley, Henry Annandale | This work is licensed under CC Attribution-NoDerivatives 4.0
Submitted: 17 December 2014 | Published: 15 June 2015

About the author(s)

Geoff Brown, Department of Production Animal Studies, University of Pretoria, South Africa
Estelle H. Venter, Department of Veterinary Tropical Diseases, University of Pretoria, South Africa
Paul Morley, Diagnostic Medicine Center, Colorado State University, United States
Henry Annandale, Onderstepoort Veterinary Academic Hospital, University of Pretoria, South Africa


Share this article

Bookmark and Share

Abstract

Rift Valley fever (RVF) is an arthropod-borne viral disease of importance in livestock and humans. Epidemics occur periodically in domestic ruminants. People in contact with infected livestock may develop disease that varies from mild flu-like symptoms to fatal viraemia. Livestock vaccination may assist in disease control. Rift Valley fever virus (RVFV) Clone 13 is a relatively new vaccine against RVF, derived from an avirulent natural mutant strain of RVFV, and has been shown to confer protective immunity against experimental infection with RVFV. The hypothesis tested in the current trial was that rams vaccinated with RVFV Clone 13 vaccine would not experience a reduction in semen quality (measured by evaluating the percentage progressively motile and percentage morphologically normal spermatozoa in successive ejaculates) relative to unvaccinated control animals. Ram lambs were screened for antibodies to RVFV using a serum neutralisation test. Animals without detectable antibodies (n = 23) were randomly allocated to either a test group (n = 12) or a control group (n = 11). Animals in the test group were vaccinated with RVFV Clone 13 vaccine. Daily rectal temperature measurements and weekly semen and blood samples were taken from all animals. Seven animals were eliminated from the statistical analysis because of potential confounding factors. Logistic regression analysis was performed on data gathered from the remaining animals to determine whether an association existed between animal group, rectal temperature and semen quality parameters. No correlation existed between the treatment group and values obtained for the semen quality parameters measured. There was no statistically significant post-vaccination decline in the percentage of live morphologically normal spermatozoa, or the percentage of progressively motile spermatozoa, either when assessed amongst all animals or when assessed within individual groups. A repeat study with a larger sample size and a more comprehensive pre-screening process may be indicated to avoid the inclusion of unsuitable animals.


Keywords

Rift Valley fever, vaccine, clone 13, rams, sheep, semen quality

Metrics

Total abstract views: 2314
Total article views: 4290


Crossref Citations

No related citations found.