Original Research

Detection of Anaplasma antibodies in wildlife and domestic species in wildlife-livestock interface areas of Kenya by major surface protein 5 competitive inhibition enzyme-linked immunosorbent assay

J.J.N. Ngeranwa, S.P. Shompole, E.H. Venter, A. Wambugu, J.E. Crafford, B.L. Penzhorn
Onderstepoort Journal of Veterinary Research | Vol 75, No 3 | a95 | DOI: https://doi.org/10.4102/ojvr.v75i3.95 | © 2008 J.J.N. Ngeranwa, S.P. Shompole, E.H. Venter, A. Wambugu, J.E. Crafford, B.L. Penzhorn | This work is licensed under CC Attribution 4.0
Submitted: 10 September 2008 | Published: 10 September 2008

About the author(s)

J.J.N. Ngeranwa,
S.P. Shompole,
E.H. Venter,
A. Wambugu,
J.E. Crafford,
B.L. Penzhorn,

Full Text:

PDF (201KB)

Abstract

The seroprevalence of Anaplasma antibodies in wildlife (eland, blue wildebeest, kongoni, impala, Thomson's gazelle, Grant's gazelle, giraffe and plains zebra) and domestic animal (cattle, sheep and goat) populations was studied in wildlife / livestock interface areas of Kenya. Serum samples were analyzed by competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA), using a recombinant antigen (MSP-5) from Anaplasma marginale surface membrane. A monoclonal antibody, FC-16, was used as the primary antibody, while anti-mouse conjugated to horseradish peroxidase was used as the secondary antibody. The results indicate a high seroprevalence in both wildlife and livestock populations, in contrast to earlier reports from Kenya, which indicated a low seroprevalence. The differences are attributed to the accurate analytical method used (CI-ELISA), as compared with agglutination techniques, clinical signs and microscopy employed by the earlier workers.

Keywords

No related keywords in the metadata.

Metrics

Total abstract views: 5055
Total article views: 4626

 

Crossref Citations

1. Molecular epidemiology of bovine anaplasmosis with a particular focus in Mexico
Sergio D. Rodríguez, Miguel Ángel García Ortiz, Rafael Jiménez Ocampo, Carlos A. Vega y Murguía
Infection, Genetics and Evolution  vol: 9  issue: 6  first page: 1092  year: 2009  
doi: 10.1016/j.meegid.2009.09.007

2. Expanding the scope of challenges to human-wildlife coexistence, and the implications for conservation: a case study of Laikipia, Kenya
Valerie A. Benka
Human Dimensions of Wildlife  vol: 28  issue: 6  first page: 585  year: 2023  
doi: 10.1080/10871209.2022.2136421

3. Tick-borne blood parasites in nyala (Tragelaphus angasii, Gray 1849) from KwaZulu-Natal, South Africa
S. Pfitzer, M.C. Oosthuizen, A.-M. Bosman, I. Vorster, B.L. Penzhorn
Veterinary Parasitology  vol: 176  issue: 2-3  first page: 126  year: 2011  
doi: 10.1016/j.vetpar.2010.11.006

4. Prevalence of hemoprotozoan parasites in small ruminants along a human-livestock-wildlife interface in western Uganda
Keneth Iceland Kasozi, Monica Namayanja, Alex Kiarie Gaithuma, Michael Mahero, Enock Matovu, Junya Yamagishi, Chihiro Sugimoto, Ewan MacLeod
Veterinary Parasitology: Regional Studies and Reports  vol: 17  first page: 100309  year: 2019  
doi: 10.1016/j.vprsr.2019.100309

5. Zebra stripes: the questions raised by the answers
Hamish M. Ireland, Graeme D. Ruxton
Biological Reviews  vol: 100  issue: 6  first page: 2660  year: 2025  
doi: 10.1111/brv.70063

6. Seroprevalence of Anaplasma marginale and Babesia bigemina infections and associated risk factors in Machakos County, Kenya
F. D. Wesonga, J. M. Gachohi, P. M. Kitala, J. M. Gathuma, M. J. Njenga
Tropical Animal Health and Production  vol: 49  issue: 2  first page: 265  year: 2017  
doi: 10.1007/s11250-016-1187-2

7. Anaplasma and Theileria Pathogens in Cattle of Lambwe Valley, Kenya: A Case for Pro-Active Surveillance in the Wildlife–Livestock Interface
Michael N. Okal, Brenda Kisia Odhiambo, Peter Otieno, Joel L. Bargul, Daniel Masiga, Jandouwe Villinger, Shewit Kalayou
Microorganisms  vol: 8  issue: 11  first page: 1830  year: 2020  
doi: 10.3390/microorganisms8111830

8. Identification of novel Theileria genotypes from Grant's gazelle
Janis Hooge, Laryssa Howe, Vanessa O. Ezenwa
International Journal for Parasitology: Parasites and Wildlife  vol: 4  issue: 2  first page: 239  year: 2015  
doi: 10.1016/j.ijppaw.2015.04.004

9. Survey of Anaplasmataceae bacteria in sheep from Senegal
Mamadou Lamine Djiba, Oleg Mediannikov, Mbaye Mbengue, Yaya Thiongane, Jean-François Molez, Momar Talla Seck, Florence Fenollar, Didier Raoult, Mady Ndiaye
Tropical Animal Health and Production  vol: 45  issue: 7  first page: 1557  year: 2013  
doi: 10.1007/s11250-013-0399-y

10. Genetic characterization and phylogenetic of Anaplasma capra in Persian onagers (Equus hemionus onager)
Hamid Staji, Mohammadhasan Yousefi, Mahmoud Ahmadi Hamedani, Iradj Ashrafi Tamai, Sahar Ghaffari Khaligh
Veterinary Microbiology  vol: 261  first page: 109199  year: 2021  
doi: 10.1016/j.vetmic.2021.109199