Email this article 
Hide
Show all
Original Research
ln vitro isolation of Ehrlichia ruminantium from ovine blood into lxodes scapularis (lDE8) cell cultures
Onderstepoort Journal of Veterinary Research | Vol 75, No 2 | a10 |
DOI: https://doi.org/10.4102/ojvr.v75i2.10
| © 2008 E. Zweygarth, A. I. Josemans, H. C. Steyn
| This work is licensed under CC Attribution 4.0
Submitted: 30 August 2008 | Published: 31 August 2008
Submitted: 30 August 2008 | Published: 31 August 2008
About the author(s)
E. Zweygarth,, South AfricaA. I. Josemans,
H. C. Steyn,
Full Text:
PDF (198KB)Abstract
Four stocks of Ehrlichiar uminantium (Welgevonden, Ball3, Nonile and Blaauwkrans), the causative agent of heartwater in domestic ruminants, were isolated into lxodes capularis (lDE8) tick cells using the leukocyte fraction of the blood of infected sheep. Organisms of two of the E. ruminantrum stocks (Welgevonden and Blaauwkrans) propagated in IDEB cells were also successfully used to infect bovine endothelial cells. All stocks were successfully propagated in IDEB cells using Dulbecco's modified Eagle's medium nutrient mixture Ham F-12c ontaining 10% foetal bovine serum (FBS). The technique should be included in any attempt to isolate uncharacterized E. ruminantium stocks.
Keywords
No related keywords in the metadata.
Metrics
Total abstract views: 5525Total article views: 3877
Crossref Citations
1. Transmission of Anaplasma phagocytophilum from endothelial cells to peripheral granulocytes in vitro under shear flow conditions
Jinyong Wang, Viktor Dyachenko, Ulrike G. Munderloh, Reinhard K. Straubinger
Medical Microbiology and Immunology vol: 204 issue: 5 first page: 593 year: 2015
doi: 10.1007/s00430-015-0387-0
2. In vitro propagation and genome sequencing of three ‘atypical’ Ehrlichia ruminantium isolates
Junita Liebenberg, Helena C. Steyn, Antoinette I. Josemans, Erika Faber, Erich Zweygarth
Onderstepoort Journal of Veterinary Research vol: 87 issue: 1 year: 2020
doi: 10.4102/ojvr.v87i1.1769