Original Research

ln vitro isolation of Ehrlichia ruminantium from ovine blood into lxodes scapularis (lDE8) cell cultures

E. Zweygarth, A. I. Josemans, H. C. Steyn
Onderstepoort Journal of Veterinary Research | Vol 75, No 2 | a10 | DOI: https://doi.org/10.4102/ojvr.v75i2.10 | © 2008 E. Zweygarth, A. I. Josemans, H. C. Steyn | This work is licensed under CC Attribution-NoDerivatives 4.0
Submitted: 30 August 2008 | Published: 31 August 2008

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E. Zweygarth,, South Africa
A. I. Josemans,
H. C. Steyn,

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Abstract

Four stocks of Ehrlichiar uminantium (Welgevonden, Ball3, Nonile and Blaauwkrans), the causative agent of heartwater in domestic ruminants, were isolated into lxodes capularis (lDE8) tick cells using the leukocyte fraction of the blood of infected sheep. Organisms of two of the E. ruminantrum stocks (Welgevonden and Blaauwkrans) propagated in IDEB cells were also successfully used to infect bovine endothelial cells. All stocks were successfully propagated in IDEB cells using Dulbecco's modified Eagle's medium nutrient mixture Ham F-12c ontaining 10% foetal bovine serum (FBS). The technique should be included in any attempt to isolate uncharacterized E. ruminantium stocks.

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