Original Research

Comparison of nucleotide sequences of recent and previous lineages of peste-des-petits-ruminants viruses of sheep and goats in Nigeria

Samuel Mantip, Melvyn Quan, David Shamaki, Moritz van Vuuren
Onderstepoort Journal of Veterinary Research | Vol 83, No 1 | a1163 | DOI: https://doi.org/10.4102/ojvr.v83i1.1163 | © 2016 Samuel Mantip, Melvyn Quan, David Shamaki, Moritz van Vuuren | This work is licensed under CC Attribution-NoDerivatives 4.0
Submitted: 31 January 2016 | Published: 31 August 2016

About the author(s)

Samuel Mantip, Department of Veterinary Tropical Diseases, University of Pretoria; Viral Research Division, National Veterinary Research Institute, South Africa
Melvyn Quan, Department of Veterinary Tropical Diseases, University of Pretoria, South Africa
David Shamaki, Viral Research Division, National Veterinary Research Institute, South Africa
Moritz van Vuuren, Department of Veterinary Tropical Diseases, University of Pretoria, South Africa


Share this article

Bookmark and Share

Abstract

Peste-des-petits-ruminants virus (PPRV) is a highly contagious, fatal and economically important viral disease of small ruminants that is still endemic and militates against the production of sheep and goats in endemic areas of the world. The aim of this study was to describe the viral strains within the country. This was carried out by collecting tissue and swab samples from sheep and goats in various agro-ecological zones of Nigeria. The phylogeny of archived PPRV strains or isolates and those circulating and causing recent outbreaks was determined by sequencing of the nucleoprotein (N)-gene. Twenty tissue and swab samples from apparently healthy and sick sheep and goats were collected randomly from 18 states, namely 3 states in each of the 6 agro-ecological zones visited. A total of 360 samples were collected. A total of 35 samples of 360 (9.7%) tested positive by reverse transcriptase–polymerase chain reaction, of which 25 were from oculo-nasal swabs and 10 were from tissue samples. Neighbour-joining phylogenetic analysis using Phylogenetic Analysis Using Parsimony (PAUP) identified four different lineages, that is, lineages I, II, III and IV. Interestingly, the Nigerian strains described in this study grouped in two separate major lineages, that is, lineages II and IV. Strains from Sokoto, Oyo, Plateau and Ondo states grouped according to the historical distribution of PPRV together with the Nigerian 75/1 strain of lineage II, while other strains from Sokoto, Oyo, Plateau, Akwa-Ibom, Adamawa, Kaduna, Lagos, Bauchi, Niger and Kano states grouped together with the East African and Asian strains of lineage IV. This finding confirms that both lineage II and IV strains of PPRV are circulating in Nigeria. Previously, only strains of lineage II were found to be present in the country.


Keywords

Peste-des-petits-ruminants-virus; Nucleoprotein (N) gene; Sheep; Goats

Metrics

Total abstract views: 1527
Total article views: 2057

 

Crossref Citations

1. First genetic characterization of Peste des Petits Ruminants from Niger: On the advancing front of the Asian virus lineage
Kadidia Tounkara, Arnaud Bataille, Caroline M. Adombi, Issoufou Maikano, Gamatié Djibo, Tirumala Bharani K. Settypalli, Angelika Loitsch, Adama Diallo, Geneviève Libeau
Transboundary and Emerging Diseases  year: 2018  
doi: 10.1111/tbed.12901