Email this article 
Hide
Show all
Original Research
Comparison of nucleotide sequences of recent and previous lineages of peste-des-petits-ruminants viruses of sheep and goats in Nigeria
Submitted: 31 January 2016 | Published: 31 August 2016
About the author(s)
Samuel Mantip, Department of Veterinary Tropical Diseases, University of Pretoria; Viral Research Division, National Veterinary Research Institute, South AfricaMelvyn Quan, Department of Veterinary Tropical Diseases, University of Pretoria, South Africa
David Shamaki, Viral Research Division, National Veterinary Research Institute, South Africa
Moritz van Vuuren, Department of Veterinary Tropical Diseases, University of Pretoria, South Africa
Abstract
Peste-des-petits-ruminants virus (PPRV) is a highly contagious, fatal and economically important viral disease of small ruminants that is still endemic and militates against the production of sheep and goats in endemic areas of the world. The aim of this study was to describe the viral strains within the country. This was carried out by collecting tissue and swab samples from sheep and goats in various agro-ecological zones of Nigeria. The phylogeny of archived PPRV strains or isolates and those circulating and causing recent outbreaks was determined by sequencing of the nucleoprotein (N)-gene. Twenty tissue and swab samples from apparently healthy and sick sheep and goats were collected randomly from 18 states, namely 3 states in each of the 6 agro-ecological zones visited. A total of 360 samples were collected. A total of 35 samples of 360 (9.7%) tested positive by reverse transcriptase–polymerase chain reaction, of which 25 were from oculo-nasal swabs and 10 were from tissue samples. Neighbour-joining phylogenetic analysis using Phylogenetic Analysis Using Parsimony (PAUP) identified four different lineages, that is, lineages I, II, III and IV. Interestingly, the Nigerian strains described in this study grouped in two separate major lineages, that is, lineages II and IV. Strains from Sokoto, Oyo, Plateau and Ondo states grouped according to the historical distribution of PPRV together with the Nigerian 75/1 strain of lineage II, while other strains from Sokoto, Oyo, Plateau, Akwa-Ibom, Adamawa, Kaduna, Lagos, Bauchi, Niger and Kano states grouped together with the East African and Asian strains of lineage IV. This finding confirms that both lineage II and IV strains of PPRV are circulating in Nigeria. Previously, only strains of lineage II were found to be present in the country.
Keywords
Metrics
Total abstract views: 7124Total article views: 6534
Crossref Citations
1. Retrospective Characterization of Initial Peste des petits ruminants Outbreaks (2008–2012) in the Democratic Republic of the Congo
Leopold K. Mulumba-Mfumu, Mana Mahapatra, Adama Diallo, Brian Clarke, Augustin Twabela, Jean Pierre Matondo-Lusala, Felix Njeumi, Satya Parida
Viruses vol: 13 issue: 12 first page: 2373 year: 2021
doi: 10.3390/v13122373
2. Peste des petits ruminants in Africa: a review of currently available molecular epidemiological data, 2020
William G. Dundon, Adama Diallo, Giovanni Cattoli
Archives of Virology vol: 165 issue: 10 first page: 2147 year: 2020
doi: 10.1007/s00705-020-04732-1
3. Peste des petits ruminants in Africa: Meta-analysis of the virus isolation in molecular epidemiology studies
Samuel E. Mantip, David Shamaki, Souabou Farougou
Onderstepoort Journal of Veterinary Research vol: 86 issue: 1 year: 2019
doi: 10.4102/ojvr.v86i1.1677
4. First genetic characterization of Peste des Petits Ruminants from Niger: On the advancing front of the Asian virus lineage
Kadidia Tounkara, Arnaud Bataille, Caroline M. Adombi, Issoufou Maikano, Gamatié Djibo, Tirumala Bharani K. Settypalli, Angelika Loitsch, Adama Diallo, Geneviève Libeau
Transboundary and Emerging Diseases vol: 65 issue: 5 first page: 1145 year: 2018
doi: 10.1111/tbed.12901
5. Epidemiology of Peste des Petits Ruminants in Nigeria: A Review
Daniel Esonu, Bryony Armson, Mohammed Babashani, Ruth Alafiatayo, Abel B. Ekiri, Alasdair J. C. Cook
Frontiers in Veterinary Science vol: 9 year: 2022
doi: 10.3389/fvets.2022.898485
6. Mapping risk areas for the occurrence of Peste des petits ruminants in the Plateau, Bauchi, and Kano states in Nigeria by combining expert knowledge and field surveys on animal mobility
Sandra I. Ijoma, Asma Mesdour, Rayyanu A. Usman, Muhammad-Bashir Bolajoko, Chika Nwosuh, Marion Bordier, Arnaud Bataille, Adeiza M. Abdulrahman, Wesley D. Nafarnda, Andrea Apolloni, Elena Arsevska
Preventive Veterinary Medicine vol: 249 first page: 106787 year: 2026
doi: 10.1016/j.prevetmed.2026.106787
7. Combining market surveys and participative approaches to map small ruminant mobility in three selected states in northern Nigeria
Sandra I. Ijoma, Asma Mesdour, Muhammad-Bashir Bolajoko, Chika Nwosuh, Marion Bordier, Arnaud Bataille, Adeiza M. Abdulrahman, Wesley D. Nafarnda, Elena Arsevska, Andrea Apolloni, Clement Ameh Yaro
PLOS One vol: 20 issue: 9 first page: e0311030 year: 2025
doi: 10.1371/journal.pone.0311030
8. Molecular epidemiology of peste des petits ruminants virus in Nigeria: An update
Samuel Mantip, Anthony Sigismeau, David Shamaki, Timothy Yusuf Woma, Olivier Kwiatek, Genevieve Libeau, Souabou Farougou, Arnaud Bataille
Transboundary and Emerging Diseases vol: 69 issue: 3 first page: 1634 year: 2022
doi: 10.1111/tbed.14073
9. Persistence of the historical lineage I of West Africa against the ongoing spread of the Asian lineage of peste des petits ruminants virus
Kadidia Tounkara, Olivier Kwiatek, Cheick Abou Kounta Sidibe, Amadou Sery, Marthin Dakouo, Habib Salami, Modou Moustapha Lo, Aminata Ba, Mariame Diop, Mamadou Niang, Geneviève Libeau, Arnaud Bataille
Transboundary and Emerging Diseases vol: 68 issue: 6 first page: 3107 year: 2021
doi: 10.1111/tbed.14066
10. Wide circulation of peste des petits ruminants virus in sheep and goats across Nigeria
Samuel E. Mantip, Anthony Sigismeau, Maurice Nanven, Atuman Joel, Abayomi M. Qasim, Sada Aliyu, Ibrahim Musa, Ogechukwu Ezeanyika, Ibikunle Faramade, Garba Ahmed, Timothy Y. Woma, David Shamaki, Genevieve Libeau, Souaibou Farougou, Arnaud Bataille
Onderstepoort Journal of Veterinary Research vol: 88 issue: 1 year: 2021
doi: 10.4102/ojvr.v88i1.1899