Original Research

Anthelmintic activity of acetone extracts from South African plants used on egg hatching of Haemonchus contortus

Gerda Fouche, Bellonah M. Sakong, Olubukola T. Adenubi, Elizabeth Pauw, Tlabo Leboho, Kevin W. Wellington, Jacobus N. Eloff
Onderstepoort Journal of Veterinary Research | Vol 83, No 1 | a1164 | DOI: https://doi.org/10.4102/ojvr.v83i1.1164 | © 2016 Gerda Fouche, Bellonah M. Sakong, Olubukola T. Adenubi, Elizabeth Pauw, Tlabo Leboho, Kevin W. Wellington, Jacobus N. Eloff | This work is licensed under CC Attribution-NoDerivatives 4.0
Submitted: 02 February 2016 | Published: 29 July 2016

About the author(s)

Gerda Fouche, CSIR Biosciences, Pretoria, South Africa
Bellonah M. Sakong, Department of Paraclinical Sciences, University of Pretoria, South Africa
Olubukola T. Adenubi, Department of Paraclinical Sciences, University of Pretoria, South Africa
Elizabeth Pauw, Department of Paraclinical Sciences, University of Pretoria, Rwanda
Tlabo Leboho, CSIR Biosciences, Pretoria, South Africa
Kevin W. Wellington, CSIR Biosciences, Pretoria, South Africa
Jacobus N. Eloff, Department of Paraclinical Sciences, University of Pretoria, South Africa


Share this article

Bookmark and Share

Abstract

The nematode, Haemonchus contortus, is responsible for major economic losses in the livestock industry. The management of parasites such as H. contortus has been through the use of synthetic parasiticides. This has resulted in the presence of residues in meat and milk, which affects food safety. The development of resistance to available anthelmintics coupled with their high cost has further complicated matters. This has led to the investigation of alternative methods to manage nematodes, including the use of plants and plant extracts as a potential source of novel anthelmintics. Acetone extracts were prepared from 15 South African plant species and their anthelmintic activity determined using the egg hatch assay (EHA). The leaf extract of Cleome gynandra had the best inhibitory activity (68% ± 3%) at a concentration of 2.5 mg/mL, followed by the stem extract of Maerua angolensis (65% ± 5%). The extracts had a relatively low toxicity on Vero cells determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) cellular assay.


Keywords

Haemonchus contortus, Anthelmintic activity, Egg hatch assay, Toxicity bioassay, Plant extracts, Acetone.

Metrics

Total abstract views: 1809
Total article views: 2397

 

Crossref Citations

1. Investigation of the acaricidal activity of the acetone and ethanol extracts of 12 South African plants against the adult ticks of Rhipicephalus turanicus
Gerda Fouche, Bellonah M. Sakong, Olubukola T. Adenubi, Jean Paul Dzoyem, Vinny Naidoo, Tlabo Leboho, Kevin W. Wellington, Jacobus N. Eloff
Onderstepoort Journal of Veterinary Research  vol: 84  issue: 1  year: 2017  
doi: 10.4102/ojvr.v84i1.1523