Original Research

The fluorescent detection of Paradiplozoon sp. (Monogenea: Diplozoidae) attachment clamps' sclerites and integumental proteins : research communication

S.J. Milne, A. Avenant-Oldewage
Onderstepoort Journal of Veterinary Research | Vol 73, No 2 | a161 | DOI: https://doi.org/10.4102/ojvr.v73i2.161 | © 2006 S.J. Milne, A. Avenant-Oldewage | This work is licensed under CC Attribution-NoDerivatives 4.0
Submitted: 13 September 2006 | Published: 13 September 2006

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S.J. Milne,
A. Avenant-Oldewage,

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Abstract

In the genus Paradiplozoon, few hard structures are present therefore most of the taxonomic studies have focused on the unique ultrastructure of the sclerites. Alcohol-fixed specimens were transferred to BSA-saline for 5 min. before staining indefinitely with 5 µl WGA-TRX, 5 µl SYTO 9 and 5 µl of Cal co fluor White M2R. Rhodamine, Fluorescein and DAPI bandpass filters on the microscope enabled selective light wavelength illumination of the three flourochromes by a mercury light source. This method provided an easy and rapid methodology to show the internal sclerites of attachment clamps. It is suitable for alcohol preserved specimens and may have additional applications in other helminth organisms.

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