Original Research

The phytochemical composition and in vitro antiviral activity of decoctions from galls of Guiera senegalensis J.F. Gmel. (Combretaceae) and their relative non-toxicity for chickens

C.E. Lamien, A. Meda, E. Couacy-Hymann, A.G. Ouedraogo, O.G. Nacoulma
Onderstepoort Journal of Veterinary Research | Vol 72, No 2 | a206 | DOI: https://doi.org/10.4102/ojvr.v72i2.206 | © 2005 C.E. Lamien, A. Meda, E. Couacy-Hymann, A.G. Ouedraogo, O.G. Nacoulma | This work is licensed under CC Attribution-NoDerivatives 4.0
Submitted: 15 September 2005 | Published: 17 September 2005

About the author(s)

C.E. Lamien,
A. Meda,
E. Couacy-Hymann,
A.G. Ouedraogo,
O.G. Nacoulma,

Full Text:

PDF (55KB)

Share this article

Bookmark and Share

Abstract

Aqueous decoctions obtained from the galls of Guiera senegalensis were screened to determine their phytochemical composition and in vitro antiviral activity against fowlpox virus. In addition, we wanted to investigate the toxic effects, if any, of crude extracts in chickens. Steroids as well as cardiac glycosides not previously reported, an alkaloid, polyphenols and saponins were detected in the various fractions of organic solvents used for extracting the decoctions. Antiviral activity was determined by cytopathic effect inhibition assay in primary chicken embryo skin cells. The 50 % inhibitory concentration (EC50) was shown to be 15.6 µg/ml. Toxicity for cells was established by determining the 50 % cytotoxic concentration (CCy50). A value of 90 µg/ml and a selectivity index (CCy50/EC50) of 5.8 were obtained. In vivo studies of toxicity were performed in chickens that were dosed orally with decoctions of several concentrations for 2 weeks and then monitored for 3 months. No significant changes in several blood chemical parameters were obtained, except for a significant decline in SGOT levels in birds dosed with 100 mg/kg. These levels were nevertheless within the accepted normal range. The findings suggest that aqueous decoctions of galls from G. senegalensis are non-toxic for chickens when administered orally, even at a daily dose of 100 mg/kg for 14 days.

Keywords

No related keywords in the metadata.

Metrics

Total abstract views: 2232
Total article views: 3405

 

Crossref Citations

1. Ethnopharmacological Survey of Plants Used in the Traditional Treatment of Gastrointestinal Pain, Inflammation and Diarrhea in Africa: Future Perspectives for Integration into Modern Medicine
Timo Stark, Dorah Mtui, Onesmo Balemba
Animals  vol: 3  issue: 1  first page: 158  year: 2013  
doi: 10.3390/ani3010158