Original Research

Hepatoprotective effects of leaf extract of Annona senegalensis against aflatoxin B1 toxicity in rats

Rhulani Makhuvele, Kenn Foubert, Nina Hermans, Luc Pieters, Luc Verschaeve, Esameldin Elgorashi
Onderstepoort Journal of Veterinary Research | Vol 91, No 1 | a2133 | DOI: https://doi.org/10.4102/ojvr.v91i1.2133 | © 2024 Rhulani Makhuvele, Kenn Foubert, Nina Hermans, Luc Pieters, Luc Verschaeve, Esameldin Elgorashi | This work is licensed under CC Attribution 4.0
Submitted: 28 July 2023 | Published: 11 March 2024

About the author(s)

Rhulani Makhuvele, Department of Toxicology and Ethnoveterinary Medicine, Faculty of Public Health and Zoonoses, Agricultural Research Council-Onderstepoort Veterinary Research, Onderstepoort, Pretoria, South Africa; and, Department of Paraclinical Sciences, Faculty of Veterinary Sciences, University of Pretoria, Pretoria, South Africa
Kenn Foubert, Natural Products and Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp – Wilrijk, Belgium
Nina Hermans, Natural Products and Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp – Wilrijk, Belgium
Luc Pieters, Natural Products and Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp – Wilrijk, Belgium
Luc Verschaeve, Department of Risk and Health Impact Assessment, Sciensano (formerly Scientific Institute of Public Health), Brussels, Belgium; and, Department of Biomedical Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp – Wilrijk, Belgium
Esameldin Elgorashi, Department of Toxicology and Ethnoveterinary Medicine, Faculty of Public Health and Zoonoses, Agricultural Research Council-Onderstepoort Veterinary Research, Onderstepoort, Pretoria, South Africa; and, Department of Paraclinical Sciences, Faculty of Veterinary Sciences, University of Pretoria, Pretoria, South Africa

Abstract

Global aflatoxin contamination of agricultural commodities is of the most concern in food safety and quality. This study investigated the hepatoprotective effect of 80% methanolic leaf extract of Annona senegalensis against aflatoxin B1 (AFB1)-induced toxicity in rats. A. senegalensis has shown to inhibit genotoxicity of aflatoxin B1 in vitro. The rats were divided into six groups including untreated control, aflatoxin B1 only (negative control); curcumin (positive control; 10 mg/kg); and three groups receiving different doses (100 mg/kg, 200 mg/kg, and 300 mg/kg) of A. senegalensis extract. The rats received treatment (with the exception of untreated group) for 7 days prior to intoxication with aflatoxin B1. Serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, and creatinine were measured. Hepatic tissues were analysed for histological alterations. Administration of A. senegalensis extract demonstrated hepatoprotective effects against aflatoxin B1-induced toxicity in vivo by significantly reducing the level of serum aspartate aminotransferase and alanine aminotransferase and regenerating the hepatocytes. No significant changes were observed in the levels of alkaline phosphatase, lactate dehydrogenase, and creatinine for the AFB1 intoxicated group, curcumin+AFB1 and Annona senegalensis leaf extract (ASLE)+AFB1 (100 mg/kg, 200 mg/kg, and 300 mg/kg body weight [b.w.]) treated groups. Annona senegalensis is a good candidate for hepatoprotective agents and thus its use in traditional medicine may at least in part be justified.

Contribution: The plant extract investigated in this study can be used in animal health to protect the organism from toxicity caused by mycotoxins.


Keywords

aflatoxin B1; toxicity; hepatoprotective activity; Sprague-Dawley rats, Annona senegalensis

Sustainable Development Goal

Goal 3: Good health and well-being

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