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Original Research
Molecular identification of Cordylobia anthropophaga Blanchard (Diptera: Calliphoridae) larvae collected from dogs (Canis familiaris) in Jos South, Plateau State, Nigeria
Onderstepoort Journal of Veterinary Research | Vol 79, No 1 | a349 |
DOI: https://doi.org/10.4102/ojvr.v79i1.349
| © 2012 Ndudim I. Ogo, Emmanuel Onovoh, Oluyinka O. Okubanjo, Ruth C. Galindo, Jose-Manuel P. de la Lastra, Jose de la Fuente
| This work is licensed under CC Attribution 4.0
Submitted: 03 June 2011 | Published: 07 February 2012
Submitted: 03 June 2011 | Published: 07 February 2012
About the author(s)
Ndudim I. Ogo, National Veterinary Research Institute, Vom, NigeriaEmmanuel Onovoh, National Veterinary Research Institute, Vom, Nigeria
Oluyinka O. Okubanjo, Department of Veterinary Parasitology, Ahmadu Bello University, Nigeria
Ruth C. Galindo, Instituto de Investigacion en Recursos Cinegiticos, IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain
Jose-Manuel P. de la Lastra, Instituto de Investigacion en Recursos Cinegiticos, IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain
Jose de la Fuente, Instituto de Investigacion en Recursos Cinegiticos, IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain
Abstract
Myiasis-causing larvae were extracted from dogs attending veterinary clinics in Plateau State, Nigeria and subjected to molecular analysis involving polymerase chain reaction amplification of the 28S rRNA gene of blowflies, cloning and sequencing techniques. All larvae were confirmed as Cordylobia anthropophaga Blanchard (Diptera: Calliphoridae) after the initial morphological identification. This is the first molecular identification of any myiasis-causing fly species in Nigeria and may serve as a reliable alternative to morphological identification where samples are not well preserved or difficult to identify to species level.
Keywords
Cordylobia anthropophaga; dog; molecular identification; myiasis; Nigeria
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