Original Research

Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid

Folashade Olaifa, Joseph O. Ayo, Suleiman F. Ambali, Peter I. Rekwot
Onderstepoort Journal of Veterinary Research | Vol 79, No 1 | a413 | DOI: https://doi.org/10.4102/ojvr.v79i1.413 | © 2012 Folashade Olaifa, Joseph O. Ayo, Suleiman F. Ambali, Peter I. Rekwot | This work is licensed under CC Attribution 4.0
Submitted: 11 February 2012 | Published: 05 December 2012

About the author(s)

Folashade Olaifa, Department of Physiology and Pharmacology, Ahmadu Bello University, Nigeria
Joseph O. Ayo, Department of Physiology and Pharmacology, Ahmadu Bello University, Nigeria
Suleiman F. Ambali, Department of Physiology and Pharmacology, Ahmadu Bello University, Nigeria
Peter I. Rekwot, National Animal Production Research Institute, Ahmadu Bello University, Nigeria

Abstract

Experiments were performed with the aim of investigating the effect of packing on erythrocyte osmotic fragility (EOF) and malondialdehyde (MDA) concentration in donkeys, and the effect of ascorbic acid (AA). Twelve apparently healthy donkeys raised under the traditional extensive system served as experimental subjects. Six donkeys administered orally with AA (200 mg/kg) and subjected to packing were used as experimental animals, whilst six others not administered with AA served as controls. Blood samples were collected pre- and post-packing from all the donkeys for the determination of MDA and EOF. At 0.3% Sodium Chloride (NaCl) concentration, the percentage haemolysis was 93.69% ± 2.21% in the control donkeys and the value was significantly (P < 0.05) higher than the value of 71.31% ± 8.33%, recorded in the experimental donkeys. The post-packing MDA concentration obtained in the control donkeys was 39.62 µmol ± 4.16 µmol, and was not significantly different (P > 0.05) from the value of 35.97 µmol ± 2.88 µmol recorded in the experimental donkeys. In conclusion, the increase in haemolysis obtained in the donkeys suggested that packing induced oxidative stress, which was ameliorated by AA administration.

Keywords

Ascorbic acid; donkey; haemolysis; malondialdehyde; work stress

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