Original Research

Construction and immunogenicity of a ∆apxIC/ompP2 mutant of Actinobacillus pleuropneumoniae and Haemophilus parasuis

Qiong Liu, Yuheng Gong, Yuqin Cao, Xintian Wen, Xiaobo Huang, Qigui Yan, Yong Huang, Sanjie Cao
Onderstepoort Journal of Veterinary Research | Vol 80, No 1 | a519 | DOI: https://doi.org/10.4102/ojvr.v80i1.519 | © 2013 Qiong Liu, Yuheng Gong, Yuqin Cao, Xintian Wen, Xiaobo Huang, Qigui Yan, Yong Huang, Sanjie Cao | This work is licensed under CC Attribution-NoDerivatives 4.0
Submitted: 09 September 2012 | Published: 06 March 2013

About the author(s)

Qiong Liu, College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China
Yuheng Gong, College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China
Yuqin Cao, College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China
Xintian Wen, College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China
Xiaobo Huang, College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China
Qigui Yan, College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China
Yong Huang, College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China
Sanjie Cao, College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China


Share this article

Bookmark and Share

Abstract

The apxIC genes of the Actinobacillus pleuropneumoniae serovar 5 (SC-1), encoding the ApxIactivating proteins, was deleted by a method involving sucrose counter-selection. In this study, a mutant strain of A. pleuropneumoniae (SC-1) was constructed and named DapxIC/ ompP2. The mutant strain contained foreign DNA in the deletion site of ompP2 gene of Haemophilus parasuis. It showed no haemolytic activity and lower virulence of cytotoxicity in mice compared with the parent strain, and its safety and immunogenicity were also evaluated in mice. The LD50 data shown that the mutant strain was attenuated 30-fold, compared with the parent strain (LD50 of the mutant strain and parent strain in mice were determined to be 1.0 × 107 CFU and 3.5 × 105 CFU respectively). The mutant strain that was attenuated could secrete inactivated ApxIA RTX toxins with complete antigenicity and could be used as a candidate live vaccine strain against infections of A. pleuropneumoniae and H. parasuis.

Keywords

Actinobacillus pleuropneumoniae; Haemophilus parasuis; apxIC; ompP2; immunogenicity; protective efficacy

Metrics

Total abstract views: 3692
Total article views: 7999


Crossref Citations

No related citations found.