Research Communication

Concomitant fungal and Mycobacterium bovis infections in beef cattle in Kenya

Joseph N. Kuria, Stephen M. Gathogo
Onderstepoort Journal of Veterinary Research | Vol 80, No 1 | a585 | DOI: | © 2013 Joseph N. Kuria, Stephen M. Gathogo | This work is licensed under CC Attribution 4.0
Submitted: 01 March 2013 | Published: 31 July 2013

About the author(s)

Joseph N. Kuria, Department of Veterinary Pathology, Microbiology and Parasitology, University of Nairobi, Kenya
Stephen M. Gathogo, Department of Veterinary Services, Ministry of Livestock Development, Kenya


Bovine tuberculosis is an important zoonosis and accurate diagnosis is important for its surveillance. Post-mortem diagnosis may, however, be compromised by lesions caused by other pathogens. In an investigation on its prevalence in slaughter cattle in Kenya, Mycobacterium bovis and dimorphic fungi were inadvertently identified separately or concurrently in tuberculous lesions. Beef carcasses were inspected for lesions in two abattoirs in Nairobi. Tissues with lesions were collected and transported to the laboratory. Smears of lesions were stained by acid-fast procedure and examined microscopically. Lesions were cultured in Löwenstein-Jensen (LJ) and in BBL TM Mycobacterium growth indicator tubes (MGIT) media. Mycobacteria isolates in LJ medium were identified by DNA typing. Smears of BBLTM MGIT cultures were acid-fast stained and examined microscopically. Tissue sections were stained with periodic acid-Schiff reagent before examination. Of the 929 carcasses examined, 176 had granulomatous lesions. Dimorphic fungi were detected as acid-fast negative cells in 58 (32.9; 33.5%) of the lesion smears, either alone (29.0; 16.4%) or concurrently with acid-fast bacilli (29.0; 16.4%). The fungi were also detected in some BBL TM MGIT-culturesmears and lesioned tissue sections. The fungi were identified, by means of cellular morphology, as Paracoccidioides brasiliensis and Blastomyces dermatitidis. A total of 64 isolates of mycobacteria were recovered in LJ medium, 19 of which were identified as M. bovis. The present report documents native P. brasiliensis infections outside the presumed endemic region and B. dermatitidis infections in a livestock animal. The findings further indicate the importance of dimorphic fungi as a differential diagnosis of bovine tuberculosis in the region.


Dimorphic fungi; M. bovis; infections; cattle


Total abstract views: 4309
Total article views: 9880

Crossref Citations

No related citations found.