Original Research

Haemophilus paragallinarum haemagglutinin : role in adhesion, serotyping and pathogenicity

T.G. Barnard, E. Van Heerden, R.R. Bragg, J. Albertyn
Onderstepoort Journal of Veterinary Research | Vol 75, No 1 | a83 | DOI: https://doi.org/10.4102/ojvr.v75i1.83 | © 2008 T.G. Barnard, E. Van Heerden, R.R. Bragg, J. Albertyn | This work is licensed under CC Attribution-NoDerivatives 4.0
Submitted: 10 September 2008 | Published: 10 September 2008

About the author(s)

T.G. Barnard,
E. Van Heerden,
R.R. Bragg,
J. Albertyn,

Full Text:

PDF (417KB)

Share this article

Bookmark and Share

Abstract

It is suggested that Haemophilus paragallinarum requires at least three haemagglutinins for adhesionduring infection. This paper reports the partial purification and characterization of the HA-L haemagglutininfrom H. paragallinarum strain 46-C3, a heat sensitive, trypsin sensitive haemagglutinin that hasbeen shown to be the serovar specific haemagglutinin in this organism. Using the pI and molecularmass obtained, it was shown that this protein shares similarities with other types of adhesins found in Gram-negative bacteria. The haemagglutination assay conditions were optimized at pH 7.5 at 37 °C. It was also shown that activity is enhanced by the addition of Ca2+ and Mn2+ ions.

Keywords

No related keywords in the metadata.

Metrics

Total abstract views: 2125
Total article views: 7301

 

Crossref Citations

1. Bacterial determinants of importance in the virulence of Gallibacterium anatis in poultry
Gry Persson, Anders M Bojesen
Veterinary Research  vol: 46  issue: 1  year: 2015  
doi: 10.1186/s13567-015-0206-z