Original Research

Relationship between haemoglobin concentration and packed cell volume in cattle blood samples

Paa-Kobina Turkson, Ebenezer Y. Ganyo
Onderstepoort Journal of Veterinary Research | Vol 82, No 1 | a863 | DOI: https://doi.org/10.4102/ojvr.v82i1.863 | © 2015 Paa-Kobina Turkson, Ebenezer Y. Ganyo | This work is licensed under CC Attribution 4.0
Submitted: 13 August 2014 | Published: 27 February 2015

About the author(s)

Paa-Kobina Turkson, Department of Animal Science, University of Cape Coast, Ghana
Ebenezer Y. Ganyo, Department of Biomedical and Forensic Sciences, University of Cape Coast, Ghana

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A convention that has been adopted in medicine is to estimate haemoglobin (HB) concentration as a third of packed cell volume (PCV) or vice versa. The present research set out to determine whether a proportional relationship exists between PCV and Hb concentration in cattle blood samples, and to assess the validity of the convention of estimating Hb concentration as a third of PCV. A total of 440 cattle in Ghana from four breeds (Ndama, 110; West African Short Horn, 110; Zebu, 110 and Sanga, 110) were bled for haematological analysis, specifically packed cell volume, using the microhaematocrit technique and haemoglobin concentration using the cyanmethaemoglobin method. Means, standard deviations, standard errors of mean and 95% confidence intervals were calculated. Trendline analyses generated linear regression equations from scatterplots. For all the cattle, a significant and consistent relationship (r = 0.74) was found between Hb concentration and PCV (%). This was expressed as Hb concentration (g/dL) = 0.28 PCV + 3.11. When the Hb concentration was estimated by calculating it as a third of PCV, the relationship was expressed in linear regression as Hb concentration (g/dL) = 0.83 calculated Hb + 3.11. The difference in the means of determined (12.2 g/dL) and calculated (10.9 g/dL) Hb concentrations for all cattle was significant (p < 0.001), whereas the difference in the means of determined Hb and corrected calculated Hb was not significant. In conclusion, a simplified relationship of Hb (g/dL) = (0.3 PCV) + 3 may provide a better estimate of Hb concentration from the PCV of cattle.


Packed cell volume, haemoglobin concentration, cattle, microhaematocrit


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