Original Research

Bovine intestinal cellular responses following primary and challenge infections with Calicophoron microbothrium metacercariae

N. Mavenyengwa, S. Mukaratirwa, M. Obwolo, J. Monrad
Onderstepoort Journal of Veterinary Research | Vol 75, No 2 | a9 | DOI: https://doi.org/10.4102/ojvr.v75i2.9 | © 2008 N. Mavenyengwa, S. Mukaratirwa, M. Obwolo, J. Monrad | This work is licensed under CC Attribution-NoDerivatives 4.0
Submitted: 30 August 2008 | Published: 31 August 2008

About the author(s)

N. Mavenyengwa, Faculty of Veterinary Science, Department of Paraclinical Veterinary Studies, University of Zimbabwe, Harare, Zimbabwe
S. Mukaratirwa, School of Biological and Conservation Sciences, University of KwaZulu-Natal, Westville Campus, Durban 4001
M. Obwolo, Faculty of Veterinary Science, Department of Paraclinical Veterinary Studies, University of Zimbabwe, Harare
J. Monrad, Danish Centre for Experimental Parasitology, Frederiksberg, Copenhagen, Denmark

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Abstract

This studyw as carried out to establish whether cattle can develop resistance to re-infectionby Calicophoron microbothrium by assessing the response of intestinal mucosal globule leukocytese, osinophils, mast cells and basophils, and the establishment of the parasite in the host. A total of 241-year old Tuli steers were randomly divided into four groups of six animals each and infected with C. microbothriumm etacercariae. On the first day of the study, animals in Groups I and II were immunized with 5000 metacercariae and then challenged with 15000 metacercariae on Day 150 post immunization. Animals in Group III were immunized with 15000 metacercariae at the same time that Groups I and II animals were challenged to act as a positive control group Animals in Group IV were left uninfected and acted as a negative control group. Three animals from each group were slaughtered on Day 28 post-challenge and the remainder were slaughtered on Day 42 post-challenge. The established amphistomes were recovered and histopathological and cytological examinations were done on the jejunum, duodenuma, bomasum and the rumen. The establishment rates of the challenge infection in the immunized and challenged groups were lower and ranged from 0 to 0.2% as compared to 6% from naive animals infected as positive controls. Animals immunized and then challenged with C. microbothrium had significantly higher eosinophil, mast cell and globule leukocytes counts in the intestinal mucosa (P < 0.05) as compared to those of the control group. The study indicates that cattle can develop resistance to C. microbothrium re-infection and that eosinophils and mast cells may be important cells in the rejection of the parasite.

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