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Original Research
Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2
Onderstepoort Journal of Veterinary Research | Vol 87, No 1 | a1886 |
DOI: https://doi.org/10.4102/ojvr.v87i1.1886
| © 2020 Tuba Ç. Oğuzoğlu, Seçkin Salar, Ece Adıgüzel, Cansu Demirden, Onur Ülgenalp
| This work is licensed under CC Attribution 4.0
Submitted: 29 May 2020 | Published: 11 November 2020
Submitted: 29 May 2020 | Published: 11 November 2020
About the author(s)
Tuba Ç. Oğuzoğlu, Department of Virology, Faculty of Veterinary Medicine, Ankara University, Ankara, TurkeySeçkin Salar, Department of Obstetric and Gnynecology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey
Ece Adıgüzel, Department of Virology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey
Cansu Demirden, Department of Virology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey
Onur Ülgenalp, Department of Virology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey
Abstract
In this study, positive blood and organ samples were obtained from different mixed herds of sheep and cattle against ovine herpesvirus 2 (OvHV-2) infection. Target-positive DNA was sequenced and compared with worldwide distributed OvHV-2 sequences. Tegument gene (422 base pairs) and glycoprotein B (gB) gene (2800 base pairs) amplicons of OvHV-2 genome were used for understanding of epidemiology of malignant catarrhal fever (MCF) infection in Turkey. The results of nucleotide sequencing of polymerase chain reaction (PCR) products indicated presence of sheep-associated form for MCF infection in Turkey. Although the obtained sequences were genetically different from each other, it was found that genetic variations were limited.
Keywords
malignant catarrhal fever; tegument gene; gB gene; OvHV-2; Turkey
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